Journal
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
Volume 66, Issue -, Pages 1493-1495Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1744309110036031
Keywords
transcription regulation in archaea; DNA binding; tryptophan sensing; TrpY
Funding
- National Institute for Biomedical Imaging and Bioengineering [P30-EB-09998]
- DOE [DE-FG02-87ER13731]
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TrpY regulates the transcription of the metabolically expensive tryptophan-biosynthetic operon in the thermophilic archaeon Methanothermobacter thermautotrophicus. TrpY was crystallized using the hanging-drop method with ammonium sulfate as the precipitant. The crystals belonged to the tetragonal space group P4(3)2(1)2 or P4(1)2(1)2, with unit-cell parameters a = b = 87, c = 147 A, and diffracted to 2.9 A resolution. The possible packing of molecules within the cell based on the values of the Matthews coefficient (V (M)) and analysis of the self-rotation function are consistent with the asymmetric unit being a dimer. Determining the structure of TrpY in detail will provide insight into the mechanisms of DNA binding, tryptophan sensing and transcription regulation at high temperature by this novel archaeal protein.
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