Journal
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
Volume 65, Issue -, Pages 102-104Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1744309108042309
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Funding
- FAPESP [06/00182-8, 06/01534-5, 06/60860-0, 04/08070-9]
- CNPq [154559/2006-7]
- Belgian Programme on Interuniversity Poles of Attraction initiated by the Belgian State, Prime Minister's Office, Science Policy Programming
- Actions de Recherche Concertees of the Communaute Francaise de Belgique
- Operation Televie
- Fonds National de la Recherche Scientifique, Belgium
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [06/01534-5, 06/00182-8] Funding Source: FAPESP
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Interleukin-22 (IL-22) is a pleiotropic cytokine that is involved in inflammatory responses. Human IL-22 was incubated with its soluble decoy receptor IL-22BP (IL-22 binding protein) and the IL-22 -IL-22BP complex was crystallized in hanging drops using the vapour-diffusion method. Suitable crystals were obtained from polyethylene glycol solutions and diffraction data were collected to 2.75 angstrom resolution. The crystal belonged to the tetragonal space group P41, with unit-cell parameters a = b = 67.9, c = 172.5 angstrom, and contained two IL-22-IL- 22BP complexes per asymmetric unit.
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