Crystallization and preliminary X-ray diffraction studies of a novel ferredoxin involved in the dioxygenation of carbazole by Novosphingobium sp KA1

Novosphingobium sp. KA1 uses carbazole 1,9adioxygenase (CARDO) as the first dioxygenase in its carbazole degradation pathway. The CARDO of KA1 contains a terminal oxygenase component and two electron transfer components: ferredoxin and ferredoxin reductase. In contrast to the CARDO systems of other species, the ferredoxin component of KA1 is a putidaredoxin type protein. This novel ferredoxin was crystallized at 293 K by the hanging drop vapour diffusion method using PEG MME 550 as the precipitant under anaerobic conditions. The crystals belong to space group C222(1) and diffraction data were collected to a resolution of 1.9 angstrom (the diffraction limit was 1.6 angstrom).