Journal
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
Volume 68, Issue -, Pages 124-133Publisher
WILEY-BLACKWELL
DOI: 10.1107/S0907444911052085
Keywords
radiation damage; dose rate; room temperature; protein crystallography
Funding
- National Institutes of Health (NIH) [GM065981-05A1]
- National Science Foundation (NSF)
- NIH/National Institute of General Medical Sciences under NSF [DMR-0225180]
- NIH through its National Center for Research Resources [RR-01646]
- companies of the Industrial Macromolecular Crystallography Association through a contract with Hauptman-Woodward Medical Research Institute
- US Department of Energy, Office of Science, Office of Basic Energy Science [DE-AC02-06CH11357]
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Global radiation damage to 19 thaumatin crystals has been measured using dose rates from 3 to 680 kGy s-1. At room temperature damage per unit dose appears to be roughly independent of dose rate, suggesting that the timescales for important damage processes are less than similar to 1 s. However, at T = 260 K approximately half of the global damage manifested at dose rates of similar to 10 kGy s-1 can be outrun by collecting data at 680 kGy s-1. Appreciable sample-to-sample variability in global radiation sensitivity at fixed dose rate is observed. This variability cannot be accounted for by errors in dose calculation, crystal slippage or the size of the data sets in the assay.
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