Journal
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
Volume 64, Issue -, Pages 1210-1221Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S0907444908030564
Keywords
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Funding
- Department of Energy
- Office of Biological and Environmental Research
- National Institutes of Medical Sciences
- Array BioPharma
- Chiron
- Exelixis
- Genencor
- Genentech
- HWI
- Plexxikon
- Roche
- The Scripps Research Institute
- Stanford University
- NIGMS/PSI [U54 GM074898]
- National Center for Research Resources (NCRR) [5 P41 RR001209]
- National Institutes of Health (NIH)
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Complete automation of the macromolecular crystallography experiment has been achieved at SSRL through the combination of robust mechanized experimental hardware and a flexible control system with an intuitive user interface. These highly reliable systems have enabled crystallography experiments to be carried out from the researchers' home institutions and other remote locations while retaining complete control over even the most challenging systems. A breakthrough component of the system, the Stanford Auto-Mounter (SAM), has enabled the efficient mounting of cryocooled samples without human intervention. Taking advantage of this automation, researchers have successfully screened more than 200 000 samples to select the crystals with the best diffraction quality for data collection as well as to determine optimal crystallization and cryocooling conditions. These systems, which have been deployed on all SSRL macromolecular crystallography beamlines and several beamlines worldwide, are used by more than 80 research groups in remote locations, establishing a new paradigm for macromolecular crystallography experimentation.
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