Journal
ACTA CIRURGICA BRASILEIRA
Volume 25, Issue 1, Pages 59-62Publisher
ACTA CIRURGICA BRASILEIRA
DOI: 10.1590/S0102-86502010000100014
Keywords
Oxidative Stress; Free Radicals; Protein Carbonylation; Ischemia; Reperfusion; Intestine Small; Rats
Categories
Ask authors/readers for more resources
Purpose: An experimental study was performed to investigate the use of protein carbonyl group as a specific biological marker for oxidative stress in a rat model of intestinal ischaemia-reperfusion. Methods: Twenty four male Wistar rats were randomly distributed into three groups with eight animals each: Group 1 - Control group; Group 2 - Sham; Group 3 - Intestinal ischaemia by clamping ileal branches of the superior mesenteric artery for one hour, followed by another hour of reperfusion. Blood samples were taken in order to analyze the protein carbonyl level by Slot blotting assay. Results: In group 3 a significant increase of protein carbonyl level was observed if compared to the homogenous levels of groups 1 and 2. Conclusion: From the results it may be concluded that the protein carbonylation may be used as a specific marker for measuring oxidative stress in rat intestinal reperfusion model.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available