Journal
ACTA BIOMATERIALIA
Volume 80, Issue -, Pages 154-168Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2018.09.013
Keywords
Myocardial infarction; Mesenchymal stem cells; Ventricular remodeling; Stem cell nano-patch
Funding
- National Key Research and Development Program of China [2016YFA0100900, 2016YFB0303303]
- Projects of International Cooperation and Exchanges NSFC [81820108019]
- National Nature Science Foundation of China [81530058, 81570272, 81500360, 51873157]
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Poor functional survival of the engrafted stem cells limits the therapeutic efficacy of stem-cell-based therapy for myocardial infarction (MI). Cardiac patch-based system for cardiac repair has emerged as a potential regenerative strategy for MI. This study aimed to design a cardiac patch to improve the retention of the engrafted stem cells and provide mechanical scaffold for preventing the ventricular remodeling post-MI. The patches were fabricated with electrospinning cellulose nanofibers modified with chitosan/silk fibroin (CS/SF) multilayers via layer-by-layer (LBL) coating technology. The patches engineered with adipose tissue-derived mesenchymal stem cells (AD-MSCs) (cell nano-patch) were adhered to the epicardium of the infarcted region in rat hearts. Bioluminescence imaging (BLI) revealed higher cell viability in the cell nano-patch group compared with the intra-myocardial injection group. Echocardiography demonstrated less ventricular remodeling in cell nano-patch group, with a decrease in the left ventricular end-diastolic volume and left ventricular end-systolic volume compared with the control group. Additionally, left ventricular ejection fraction and fractional shortening were elevated after cell nano-patch treatment compared with the control group. Histopathological staining demonstrated that cardiac fibrosis and apoptosis were attenuated, while local neovascularization was promoted in the cell nano-patch group. Western blot analysis illustrated that the expression of biomarkers for myocardial fibrosis (TGF-beta 1, P-smad3 and Smad3) and ventricular remodeling (BNP, beta-MHC: alpha-MHC ratio) were decreased in cell nano patch-treated hearts. This study suggests that CS/SF-modified nanofibrous patches promote the functional survival of engrafted AD-MSCs and restrain ventricular remodeling post-MI through attenuating myocardial fibrosis. (C) 2018 Published by Elsevier Ltd on behalf of Acta Materialia Inc.
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