Journal
INDIAN JOURNAL OF GENETICS AND PLANT BREEDING
Volume 75, Issue 3, Pages 349-356Publisher
INDIAN SOC GENET PLANT BREEDING
DOI: 10.5958/0975-6906.2015.00055.3
Keywords
Indian mustard; seed weight; SHB1; KLUH; transformation
Categories
Funding
- ICAR
- IARI
Ask authors/readers for more resources
Seed size is a highly heritable trait in mustard and increasing seed size could improve crop productivity. Here we report the effect of overexpression of two Arabidopsis genes SHB1 and KLUH on seed weight and other yield contributing traits in Brassica juncea. KLUH gene was expressed under its own promoter, whereas the constitutive HPL promoter drove SHB1. Transgenic status was confirmed through PCR, RT-PCR and Southern analyses. The three SHB1 events contained single copy of transgene, whereas 12 KLUH events carried 1-4 copies of transgenes. All transgenics were fertile and comparable to non-transgenic counterpart for most of the agronomic traits in both T-o and T1 generations. Seed size and seed weight in SHB1transgenics were 30-40% higher than the control, whereas one KLUH event showed significant increase in seed weight. Constitutive overexpression of SHB1 in B. juncea had undesirable pleiotropic effects that were comparable to the Arabidopsis lines overexpressing IKU2.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available