Journal
ACTA BIOMATERIALIA
Volume 10, Issue 6, Pages 2653-2662Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2014.02.020
Keywords
Cellular uptake; Multilayer capsules; Layer-by-layer; Biomaterials
Funding
- Fundacao para a Ciencia e Tecnologia [SFRH/BD/61126/2009]
- Fundo Social Europeu
- EU 7th Framework Programme [REGPOT-CT2012-316331-POLARIS]
- EU through the European Regional Development Fund (ERDF)
- MINECO [MAT-2010-15982, MAT2010-15310, PRI-PI-BAR-2011-1403, MAT2012-38043]
- JCyL [VA049A11, VA152A12, VA155A12]
- CIBER-BBN
- Instituto de Salud Carlos III under the Network Center of Regenerative Medicine and Cellular Therapy of Castilla and Leon
- Programa Diferencial de Potencial Humano
- Fundação para a Ciência e a Tecnologia [SFRH/BD/61126/2009] Funding Source: FCT
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Multilayered microcapsules of chitosan and biomimetic elastin-like recombinamers (ELRs) were prepared envisaging the intracellular delivery of active agents. Two ELRs containing either a bioactive RGD sequence or a scrambled non-functional RDG were used to construct two types of functionalized polymeric microcapsules, both of spherical shape 4 pm in diameter. Cell viability studies with human mesenchymal stem cells (hMSCs) were performed using microcapsule/cell ratios between 5:1 and 100:1. After 3 and 72 h of co-incubation, no signs of cytotoxicity were found, but cells incubated with RGD-functionalized microcapsules exhibited higher viability values than RDG cells. The internalization efficacy and bioavailability of encapsulated DQ-ovalbumin were assessed by monitoring the fluorescence changes in the cargo. The data show that surface functionalization did not significantly influence internalization by hMSCs, but the bioavailability of DQ-ovalbumin degraded faster when encapsulated within RGD-functionalized microcapsules. The microcapsules developed show promise for intracellular drug delivery with increased drug efficacy. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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