Journal
ACTA BIOMATERIALIA
Volume 8, Issue 8, Pages 2978-2985Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2012.04.027
Keywords
Virus; RGD insertion; Layer-by-layer assembly; Stem cell; Turnip yellow mosaic virus
Funding
- US NSF [CHE-0748690]
- Alfred P. Sloan Scholarship
- Camille Dreyfus Teacher Scholar Award
- W.M. Keck Foundation
- Emile F. Pernot Distinguished Professorship endowment
- N.L. Tartar Trust
- Oregon State University Agricultural Experiment Station
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0748690] Funding Source: National Science Foundation
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Turnip yellow mosaic virus (TYMV) is a stable 28 nm icosahedral plant virus that can be isolated in gram quantities. In order to study the polyvalent effect of Arg-Gly-Asp (RGD) clustering on the response of bone marrow stem cells (BMSCs), an RGD motif was genetically displayed on the coat protein of the TYMV capsid. Composite films composed of either wild-type TYMV or TYMV-RGD44, in combination with poly(allylamine hydrochloride) (PAH), were fabricated by a layer-by-layer adsorption of virus and PAH. The deposition process was studied by quartz crystal microbalance, UV-visible spectroscopy and atomic force microscopy. BMSC adhesion assays showed enhanced cell adhesion and spreading on TYMV-RGD44 coated substrates compared to native TYMV. These results demonstrate the potential of TYMV as a viable scaffold for bioactive peptide display and cell culturing studies. (c) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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