Journal
ACTA BIOMATERIALIA
Volume 7, Issue 3, Pages 1084-1093Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2010.10.022
Keywords
Collagen scaffold; Vascular endothelial growth factor; Degradation; Cellularization; Angiogenesis
Funding
- National Natural Science Foundation of China [30688002, 30930032]
- Chinese Academy of Sciences [KSCX2-YW-R-133, KSCX2-YW-G-047, KJCX2-YW-L08]
- K.C. Wang Education Foundation
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Much research has focused on developing vascular endothelial growth factor (VEGF) delivery systems to enhance angiogenesis in wound repair and in tissue engineering. Collagen can be used as a delivery system because of its biocompatibility, but its fast degradation rate and limited affinity with growth factors are disadvantageous for maintaining a sufficient growth factor concentration at injury sites. To enhance VEGF binding to collagen scaffolds and reduce the collagen degradation rate we found a simple way to modify porous collagen scaffolds by chemical addition of sulfhydryl groups, which then allow both cross-linking of the collagen fibers with each other and the immobilization of more VEGF in the scaffold after treatment with sulfo-SMCC. We demonstrated that cross-linking led to a slower degradation rate of the collagen scaffolds, while cellularization was improved by both cross-linking and the presence of VEGF. On the other hand, angiogenesis was increased only moderately by cross-linking, but significantly more by the presence of immobilized VEGF. We conclude that collagen scaffolds chemically conjugated to VEGF by Traut's reagent and sulfo-SMCC is an effective delivery system in wound repair and tissue engineering. (C) 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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