Journal
ACTA BIOMATERIALIA
Volume 6, Issue 10, Pages 4118-4126Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2010.04.029
Keywords
Stem cell encapsulation; Osteogenic differentiation; Alginate hydrogel; Calcium phosphate cement scaffold; Bone marrow mesenchymal stem cells
Funding
- NIH [R01 DE14190, R01 DE17974]
- Maryland Nano-Biotechnology Award
- Maryland Stem Cell Research Fund
- University of Maryland Dental School
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Due to its injectability and excellent osteoconductivity, calcium phosphate cement (CPC) is highly promising for orthopedic applications. However, a literature search revealed no report on human bone marrow mesenchymal stem cell (hBMSC) encapsulation in CPC for bone tissue engineering. The aim of this study was to encapsulate hBMSCs in alginate hydrogel beads and then incorporate them into CPC. CPC-chitosan and CPC-chitosan-fiber scaffolds. Chitosan and degradable fibers were used to mechanically reinforce the scaffolds After 21 days, that the percentage of live cells and the cell density of hBMSCs inside CPC-based constructs matched those in alginate without CPC, indicating that the CPC setting reaction did not harm the hBMSCs Alkaline phosphate activity Increased by 8-fold after 14 days Mineral staining, scanning electron microscopy and X-ray diffraction confirmed that apatitic mineral was deposited by the cells The amount of hBMSC-synthesized mineral in CPC-chitosan-fiber matched that in CPC without chitosan and fibers. Hence, adding chitosan and fibers, which reinforced the CPC, did not compromise hBMSC osteodifferentiation and mineral synthesis. In conclusion, hBMSCs were encapsulated in CPC and CPC-chitosan-fiber scaffolds for the first time. The encapsulated cells remained viable, osteodifferentiated and synthesized bone minerals These self-setting, hBMSC-encapsulating CPC-based constructs may be promising for bone tissue engineering applications (C) 2010 Published by Elsevier Ltd. on behalf of Acta Materialia Inc
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