Journal
ACTA BIOMATERIALIA
Volume 6, Issue 1, Pages 291-301Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2009.07.030
Keywords
Fibronectin; Extracellular matrix organization; Titanium; Surface topography; Surface energy
Funding
- CICYT [MAT2003-08165]
- Spanish Ministry of Science and Technology
- Universitat Politecnica de Catalunya (UPC)
- ICREA Funding Source: Custom
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We investigated the early events of bone matrix formation, and specifically the role of fibronectin (FN) in the initial osteoblast interaction and the subsequent organization of a provisional FN matrix on different rough titanium (Ti) surfaces. Fluorescein isothiocyanate-label led FN was preadsorbed on these surfaces and studied for its three-dimensional (3-D) organization by confocal microscopy, while its amount was quantified after NaOH extraction. An irregular pattern of adsorption with a higher amount of protein on topographic peaks than on valleys was observed and attributed to the physicochemical heterogeneity of the rough Ti surfaces. MG63 osteoblast-like cells were further cultured on FN-preadsorbed Ti surfaces and an improved initial cellular interaction was observed with increasing roughness. 3-D reconstruction of the immunofluorescence images after 4 days of incubation revealed that osteoblasts deposit FN fibrils in a specific facet-like pattern that is organized within the secreted total matrix overlying the top of the samples. The thickness of this FN layer increased when the roughness of the underlying topography was increased, but not by more than half of the total maximum peak-to-valley distance, as demonstrated with images showing simultaneous reconstruction of fluorescence and topography after 7 days of cell culture. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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