Journal
ACTA BIOMATERIALIA
Volume 6, Issue 2, Pages 494-501Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2009.07.033
Keywords
ATDC5; Chondrogenic differentiation; Poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PAMPS); Poly(dimethylacrylamide) (PDMAAm); Copolymer gels of 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) and dimethylacrylamide (DMAAm); (P(AMPS-co-DMAAm) gels)
Funding
- Takeda Science Foundation
- Ministry of Education, Science and Culture, Japan [202 40045]
Ask authors/readers for more resources
We investigated the behavior of chondrogenic ATDC5 cells on synthetic polymer gels with various charge densities: negatively charged poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PAMPS) gel, neutral poly(dimethylacrylamide) (PDMAAm) gel, and copolymer gels of 2-acrylamido-2-methyl-1-propanesulfonic acid and dimethylacrylamide P(AMPS-co-DMAAm) with different compositions (molar fractions of AMPS, F = 0.25, 0.5, 0.75). In insulin-free maintenance medium, the ATDC5 cells cultured on the highly negatively charged gels - PAMPS gel and the P(AMPS-co-DMAAm) copolymer gels (F = 0.75) - spread and became confluent at day 7, and interestingly formed nodules at day 14, expressing type 11 collagen and proteoglycan. This result demonstrates that the highly negatively charged gels can induce chondrogenic differentiation of ATDC5 cells even in insulin-free maintenance medium, in which the ATDC5 cells cultured on the standard polystyrene dish cannot differentiate into chondrocytes. In insulin-supplemented differentiation medium, ATDC5 cells cultured on the PDMAAm gel made focal adhesions, rapidly aggregated and formed large nodules within 7 days, expressing significantly greater levels of type 11 collagen and proteoglycan than cells cultured on the polystyrene dish and the negatively charged gels. These results showed that the neutral gel accelerated chondrogenic differentiation of ATDC5 cells cultured in the differentiation medium. We suggest that the highly negatively charged PAMPS gel and the neutral PDMAAm gel are interesting biomaterials for cartilage tissue engineering as a scaffold with the potential to induce chondrogenic differentiation. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available