Journal
ACTA BIOMATERIALIA
Volume 5, Issue 6, Pages 2109-2121Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2009.01.026
Keywords
Poly(butylcyanoacrylate) nanoparticles; Drug delivery system; Daunorubicin; DLKP and DLKP-A cell lines; Cytotoxicity
Funding
- EPSRC [EP/F001452/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/F001452/1] Funding Source: researchfish
Ask authors/readers for more resources
The design, preparation and characterization of poly(butylcyanoacrylate) nanoparticles as a drug-delivery system for daunorubicin is reported. A range of light scattering [photon correlation spectroscopy (PCS)], spectroscopic [H-1 nuclear magnetic resonance (H-1 NMR), Fourier transform infrared (FTIR), chromatographic [gel permeation chromatography (GPC)] and quantum chemical techniques have been employed for the physicochemical characterization of drug-loaded nanoparticles and to clarify the mechanisms of drug immobilization in the polymer matrix. The presence of daunorubicin in the polymerization medium was found to affect both the degree of polymerization and the compactness of the resulting nanoparticles. The GPC, FTIR and H-1 NMR results confirmed cytostatic immobilization in the polymer matrix, with evidence for the presence of three types of inclusion: physically entrapped, polymer-associated (due to hydrogen bonds and/or dipole-charge interactions with the polymer chains), and polymer surface-adsorbed daunorubicin. The developed colloidal delivery system has the capacity for sustained in vitro release of daunorubicin. Preliminary in vitro assays were carried out on two cell lines, DLKP and DLKP-A, which display different levels of drug resistance, to evaluate the cytotoxicity of the drug-loaded nanoparticles. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available