Journal
ACTA BIOMATERIALIA
Volume 4, Issue 5, Pages 1569-1575Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2008.02.030
Keywords
fracture healing; indentation; modulus; woven bone; cartilage
Funding
- NIH [AR053353]
Ask authors/readers for more resources
In bone fracture healing, the extent to which the injured bone regains stability and strength depends on the mechanical properties of the tissues that are formed during healing. While many techniques have been used to quantify the overall mechanical behavior of fracture calluses, few data exist on the material properties of individual callus tissues. The overall goal of this study was to quantify these material properties. Nanoindentation was performed at multiple locations across thin (200 mu m), longitudinal sections of rat fracture callus at 35 days post fracture. Following indentation, sections were stained with alizarin red S and alcian blue to obtain semi-quantitative estimates of tissue mineral content and proteoglycan content, respectively. Indentation moduli varied over three orders of magnitude (0.61-1010 MPa) throughout the callus. Much of this variation was due to the presence of multiple tissue types: the indentation moduli of granulation tissue, chondroid tissue and woven bone ranged 0.61-1.27 MPa (median = 0.99 MPa), 1.39-4.42 MPa (median = 2.89 MPa) and 26.92-1010.00 MPa (median = 132.00 MPa), respectively. In regions of alizarin red staining, the indentation modulus was correlated (r = 0.62, P = 0.04) with stain intensity, suggesting a positive correlation between modulus and mineral content in woven bone. In addition, the indentation modulus of woven bone along the periosteal aspect of the cortex increased with distance from the fracture gap (P = 0.004). These results demonstrate the usefulness of nanoindentation in characterizing the elastic properties of the heterogeneous mixture of tissues present in bone fracture callus. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available