Journal
AMERICAN JOURNAL OF PHYSICS
Volume 83, Issue 5, Pages 418-426Publisher
AMER ASSOC PHYSICS TEACHERS
DOI: 10.1119/1.4902187
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Funding
- Emmanuel College
- National Science Foundation
- NSF CAREER Award [MCB-1148818]
- NSF RUI Award [PHY-1205814]
- Division Of Physics
- Direct For Mathematical & Physical Scien [1205814] Funding Source: National Science Foundation
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A simple method for tethering microbeads using single molecules of DNA is explained. We describe how to use video microscopy and particle tracking to measure the trajectories of the microbeads' motion. The trajectories are analyzed and compared to different models of tethered particle motion. In addition, the data are used to measure the elasticity of the DNA (its spring constant), and the DNA persistence length. (C) 2015 American Association of Physics Teachers.
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