Journal
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
Volume 44, Issue 7, Pages 555-564Publisher
OXFORD UNIV PRESS
DOI: 10.1093/abbs/gms035
Keywords
cotton (Gossypium hirsutum); dirigent protein; lignin; Verticillium wilt; overexpression
Categories
Funding
- Ministry of Agriculture of China [2009ZX08009-117B, 2011ZX08009-003]
Ask authors/readers for more resources
Dirigent super-family abounds throughout the plant kingdom, especially vascular plants. To elucidate the function of cotton (Gossypium hirsutum) DIR genes in lignification, two cDNAs (designated GhDIR1 and GhDIR2) encoding putative dirigent proteins were isolated from cotton cDNA libraries. Real-time quantitative reverse transcription-polymerase chain reaction analysis revealed that GhDIR1 transcript was preferentially accumulated in cotton hypocotyls, whereas GhDIR2 was predominantly expressed in cotton fibers. Overexpression of GhDIR1 gene resulted in an increase in lignin content in transgenic cotton plants, compared with that of wild type. Histochemical assay revealed that the transgenic plants displayed more widespread lignification than that of wild type in epidermis and vascular bundle. Furthermore, the transgenic cotton plants displayed more tolerance to the infection of Verticillium dahliae. Our data suggest that GhDIR1 may be involved in cotton lignification which can block the spread of fungal pathogen V. dahliae.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available