Journal
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
Volume 42, Issue 4, Pages 274-280Publisher
OXFORD UNIV PRESS
DOI: 10.1093/abbs/gmq016
Keywords
glioma; gene therapy; GFAP promoter; Musashi1 gene promoter; hypoxia-inducible factor-binding site
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Funding
- Peking Union Medical College Hospital (PUMCH) [PUMCH-2006406]
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Glioma-specific transcription of tumor-killing genes has been exploited as a promising gene therapeutic modality in glioma patients. Musashi1 (Msi1) and GFAP gene promoters are both cancer-specific promoters. Optimized HIF-binding site (optHBS) sequence was newly found as efficient as EPO HREs used as enhancer in cancer gene therapy. We constructed 4optHBS-Msi1/GFAP promoters and tested their ability to mediate BAX expression to induce apoptosis in glioma cell lines. Our results demonstrated that 4optHBS-Msi1/GFAP promoters are apparently strong and glioma-selective promoters with potential application in targeted glioma gene therapy, and 4optHBS-Msi1/GFAP-BAX alpha are valuable tools for glioma gene therapy.
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