Journal
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
Volume 41, Issue 10, Pages 816-821Publisher
OXFORD UNIV PRESS
DOI: 10.1093/abbs/gmp074
Keywords
Fanconi anemia complementation group A (FANCA); centromere-associated protein E; yeast two-hybrid; protein interaction
Categories
Funding
- National Natural Science Foundation of China [30801329]
- Anhui Medical University [0108023101]
Ask authors/readers for more resources
This study sought to isolate and identify proteins that interact with centromere-associated protein E (CENP-E), provide new clues for exploring the function of CENP-E in cell cycle control and the pathogenesis of tumor. Yeast two-hybrid screen and regular molecular biologic techniques were undertaken to screen human HeLa cDNA library with the kinetochore binding domain of CENP-E. The bait from the C-terminus of CENP-E was created by subcloning methods to find out optimal candidate proteins that interact with the kinetochore binding domain of CENP-E. Eight novel CENP-E interacting proteins including Homo sapiens Fanconi anemia complementation group A (FANCA) were obtained. In yeast two-hybrid assay, the N-terminal 260 amino acids of FANCA were found to be necessary and sufficient for the interaction with the C-terminus of CENP-E. The interaction was confirmed by in vitro glutathione S-transferase pull-down assay and in vivo co-immunoprecipitation assay. Our finding of the interaction of CENP-E with FANCA demonstrates that CENP-E and FANCA may play important roles in the functional regulation of the mitotic checkpoint signal pathway.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available