4.8 Article

Living Nanospear for Near-Field Optical Probing

Journal

ACS NANO
Volume 12, Issue 11, Pages 10703-10711

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acsnano.8b05235

Keywords

optical trapping; subwavelength; optical probe; near field; single cells

Funding

  1. National Natural Science Foundation of China [61827822, 11874183, 11774135]
  2. Ministry of Education, Singapore [R-263-000-D11-114]

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Optical nanoprobes, designed to emit or collect light in the close proximity of a sample, have been extensively used to sense and image at nanometer resolution. However, the available nanoprobes, constructed from artificial materials, are incompatible and invasive when interfacing with biological systems. In this work, we report a fully biocompatible nanoprobe for subwavelength probing of localized fluorescence from leukemia single-cells in human blood. The bioprobe is built on a tapered fiber tip apex by optical trapping of a yeast cell (1.4 mu m radius) and a chain of Lactobacillus acidophilus cells (2 mu m length and 200 nm radius), which act as a high-aspect-ratio nano spear. Light propagating along the bionanospear can be focused into a spot with a full width at half-maximum (fwhm) of 190 nm on the surface of single cells. Fluorescence signals are detected in real time at subwavelength spatial resolution. These noninvasive and biocompatible optical probes will find applications in imaging and manipulation of biospecimens.

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