Journal
ACS NANO
Volume 8, Issue 3, Pages 2575-2583Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn406200y
Keywords
SERS; biosensor; glycerophosphoinositol; nanofabrication; label-free detection
Categories
Funding
- AIRC [11454, IG10341, PON01-00117, PON01-00862]
- FIR [RBFR12WAPY]
- EPSRC [EP/I004602/1]
- PRIN [2012CK5RPF_05]
- Engineering and Physical Sciences Research Council [EP/I004602/1] Funding Source: researchfish
- EPSRC [EP/I004602/1] Funding Source: UKRI
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Direct and quantitative detection of unlabeled glycerophosphoinositol (GroPIns), an abundant cytosolic phosphoinositide derivative, would allow rapid evaluation of several malignant cell transformations. Here we report label-free analysis of GroPIns via surface-enhanced Raman spectroscopy (SERS) with a sensitivity of 200 nM, well below its apparent concentration in cells. Crucially, our SERS substrates, based on lithographically defined gold nanofeatures, can be used to predict accurately the GroPIns concentration even in multicomponent mixtures, avoiding the preliminary separation of individual compounds. Our results represent a critical step toward the creation of SERS-based biosensor for rapid, label-free, and reproducible detection of specific molecules, overcoming limits of current experimental methods.
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