Journal
ACS NANO
Volume 8, Issue 5, Pages 5049-5060Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn5011304
Keywords
magnetotactic bacteria; nanoliposome; bioconjugation; carbodiimide chemistry; self-propelled targeted delivery; biomicrocarrier
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Funding
- Quebec Consortium for Drug Discovery (CQDM)
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Mathematics of Information Technology and Complex Systems (MITACS)
- Research Chair of Ecole Polytechnique in NanoRobotics
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The targeted and effective delivery of therapeutic agents remains an unmet goal in the field of controlled release systems. Magnetococcus marinus MC-1 magnetotactic bacteria (MTB) are investigated as potential therapeutic carriers. By combining directional magnetotaxis-microaerophilic control of these self-propelled agents, a larger amount of therapeutics can be delivered surpassing the diffusion limits of large drug molecules toward hard-to-treat hypoxic regions in solid tumors. The potential benefits of these carriers emphasize the need to develop an adequate method to attach therapeutic cargos, such as drug-loaded nanoliposomes, without substantially affecting the cell's ability to act as delivery agents. In this study, we report on a strategy for the attachment of liposomes to MTB (MTB-LP) through carbodiimide chemistry. The attachment efficacy, motility, and magnetic response of the MTB-LP were investigated. Results confirm that a substantial number of nanoliposomes (similar to 70) are efficiently linked with MTB without compromising functionality and motility. Cytotoxicity assays using three different cell types (J774, NIH/3T3, and Colo205) reveal that liposomal attachments to MTB formulation improve the biocompatibility of MTB, whereas attachment does not Interfere with liposomal uptake.
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