Journal
ACS NANO
Volume 8, Issue 10, Pages 10774-10781Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn504551d
Keywords
nanopore; ionophore; fluorescence; single-molecule; optical patch-clamping
Categories
Funding
- National Institutes of Health [R21-HG006873, R01-HG006321]
- National Science Foundation [ECCS-0335765]
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In recent years, nanopores have emerged as exceptionally promising single-molecule sensors due to their ability to detect biomolecules at subfemtomole levels in a label-free manner. Development of a high-throughput nanopore-based biosensor requires multiplexing of nanopore measurements. Electrical detection, however, poses a challenge, as each nanopore circuit must be electrically independent, which requires complex nanofluidics and embedded electrodes. Here, we present an optical method for simultaneous measurements of the ionic current across an array of solid-state nanopores, requiring no additional fabrication steps. Proof-of-principle experiments are conducted that show simultaneous optical detection and characterization of ssDNA and dsDNA using an array of pores. Through a comparison with electrical measurements, we show that optical measurements are capable of accessing equivalent transmembrane current information.
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