Journal
ACS NANO
Volume 8, Issue 1, Pages 875-884Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn405612q
Keywords
carbon; nanoelectrode; platinum; electrochemistry; intracellular measurements; brain slice; melanoma
Categories
Funding
- EPSRC
- Wellcome Trust
- Ministry of Education and Science of the Russian Federation [14.512.11.0084]
- European Research Council [ERC-2009-AdG 247143]
- Programme Retour Post-Doctorants [ANRPDOC20011-Copel]
- BBSRC [BB/J001473/1] Funding Source: UKRI
- EPSRC [EP/I007482/1] Funding Source: UKRI
- MRC [G0900613] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J001473/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/I007482/1] Funding Source: researchfish
- Medical Research Council [G0900613] Funding Source: researchfish
- Grants-in-Aid for Scientific Research [24710140] Funding Source: KAKEN
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The measurement of key molecules in individual cells with minimal disruption to the biological milieu is the next frontier in single-cell analyses. Nanoscale devices are ideal analytical tools because of their small size and their potential for high spatial and temporal resolution recordings. Here, we report the fabrication of disk-shaped carbon nanoelectrodes whose radius can be precisely tuned within the range 5-200 nm. The functionalization of the nanoelectrode with platinum allowed the monitoring of oxygen consumption outside and inside a brain slice. Furthermore, we show that nanoelectrodes of this type can be used to impale individual cells to perform electrochemical measurements within the cell with minimal disruption to cell function. These nanoelectrodes can be fabricated combined with scanning ion conductance microscopy probes, which should allow high resolution electrochemical mapping of species on or in living cells.
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