Journal
ACS NANO
Volume 7, Issue 6, Pages 4967-4976Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn4018284
Keywords
biosensor; biomarker; surface-enhanced Raman scattering; cancer; surface plasmon; blood plasma
Categories
Funding
- NSF [EPS 1003907]
- NASA-WV Space Grant Consortium
- West Virginia University Research Corporation
- West Virginia EPSCoR Office
- NIH [P30 GM103488, P30 RR 032138, R01 HL056888, RO1 CA134573]
- National Science Foundation [1102689]
- Natural Sciences and Engineering Research Council of Canada
- Fonds de la recherche sur la nature et les technologies
- Mylan Chair of Pharmacology at the WVU Health Sciences Center
- Alexander B. Osborn Hematopoietic Malignancy and Transplantation Program
- WV Research Trust Fund
- Division Of Graduate Education
- Direct For Education and Human Resources [1102689] Funding Source: National Science Foundation
- Office Of The Director
- Office of Integrative Activities [1003907] Funding Source: National Science Foundation
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A three-dimensional (3D) hierarchical plasmonic nano-architecture has been designed for a sensitive surface-enhanced Raman scattering (SERS) immunosensor for protein biomarker detection. The capture antibody molecules are immobilized on a plasmonic gold triangle nanoarray pattern. On the other hand, the detection antibody molecules are linked to the gold nanostar@Raman reporter@silica sandwich nanoparticles. When protein biomarkers are present, the sandwich nanoparticles are captured over the gold triangle nanoarray, forming a confined 3D plasmonic field, leading to the enhanced electromagnetic field in intensity and in 3D space. As a result, the Raman reporter molecules are exposed to a high density of hot spots, which amplifies the Raman signal remarkably, improving the sensitivity of the SERS immunosensor. This SERS immunosensor exhibits a wide linear range (0.1 pg/mL to 10 ng/mL) and a low limit of detection (7 fg/mL) toward human immunoglobulin G protein In the buffer solution. This biosensor has been successfully used for detection of the vascular endothelial growth factor In the human blood plasma from clinical breast cancer patient samples.
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