Journal
ACS NANO
Volume 7, Issue 5, Pages 4629-4636Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn4014388
Keywords
nanopores; silicon nitride; homopolymers; DNA; transmission electron microscopy; sequencing; translocation; single-stranded DNA; polynucleotide
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Funding
- National Science Foundation Graduate Research Fellowship (Ky.) [DGE-0822]
- National Science Foundation
- NSF-IGERT program [DGE-0221664]
- MRSEC NSF [0520020, DMR-1120901]
- NIH [R21HG004767, R01HG006879]
- Nano/Bio Interface Center through the National Science Foundation
- NSEC [DMR08-32802]
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [0520020] Funding Source: National Science Foundation
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In the last two decades, new techniques that monitor ionic current modulations as single molecules pass through a nanoscale pore have enabled numerous single-molecule studies. While biological nanopores have recently shown the ability to resolve single nucleotides within individual DNA molecules, similar developments with solid-state nanopores have lagged, due to challenges both in fabricating stable nanopores of similar dimensions as biological nanopores and in achieving sufficiently low-noise and high-bandwidth recordings. Here we show that small silicon nitride nanopores (0.8- to 2-nm diameter in 5- to 8-nm-thick membranes) can resolve differences between Ionic current signals produced by short (30 base) ssDNA homepolymers (poly(dA), poly(dc, poly(dT)), when combined with measurement electronics that allow a signal-to-noise ratio of better than 10 to be achieved at 1-MHz bandwidth. While identifying intramolecular DNA sequences with silicon nitride nanopores will require further improvements in nanopore sensitivity and noise levels, homopolymer differentiation represents an Important milestone in the development of solid-state nanopores.
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