Journal
ACS NANO
Volume 7, Issue 2, Pages 903-910Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn302322j
Keywords
structural DNA nanotechnology; molecular self-assembly; DNA origami; chip-synthesized oligonucleotides; synthetic biology; gene-synthesis; inkjet
Categories
Funding
- [NSF-EMT-0829749]
- [NSF-CCF-0835794]
- [NSF-IRES-0965965]
- [ONR-N000140910249]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0835794] Funding Source: National Science Foundation
- Office Of The Director
- Office Of Internatl Science &Engineering [1246799] Funding Source: National Science Foundation
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Although structural DNA nanotechnology, and especially scaffolded DNA origami, hold great promise for bottom-up fabrication of novel nanoscale materials and devices, concerns about scalability have tempered widespread enthusiasm. Here we report a single-pot reaction where both strands of double-stranded M13-bacteriophage DNA are simultaneously folded into two distinct shapes that then heterodimerize with high yield. The fully addressable, two-dimensional heterodimer DNA origami, with twice the surface area of standard M13 origami, formed in high yield (81% of the well-formed monomers undergo dimerization). We also report the concurrent production of entire sets of staple strands by a unique, nicking strand-displacement amplification (nSDA) involving reusable surface-bound template strands that were synthesized in situ using a custom piezoelectric inkjet system. The combination of chip-based staple strand production, double-sized origami, and high-yield one-pot assembly markedly increases the useful scale of DNA origami.
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