Journal
ACS NANO
Volume 8, Issue 1, Pages 941-949Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn405744f
Keywords
magnetic nanospheres; circulating tumor cells; rapid and efficient capture; sensitive detection
Categories
Funding
- National Basic Research Program of China (973 Program) [2011CB933600]
- 863 Program [2013AA032204]
- Science Fund for Creative Research Groups of NSFC [20921062]
- National Natural Science Foundation of China [21175100]
- Program for New Century Excellent Talents in University [NCET-10-0656]
- Administrative Committee of East Lake Hi-Tech Development Zone [[2011]137]
- 111 Project [111-2-10]
- Program for Cultivation of Inter-disciplinarily Innovative Talents of Wuhan University
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The study on circulating tumor cells (CTCs) has great significance for cancer prognosis, treatment monitoring, and metastasis diagnosis, in which isolation and enrichment of CTCs are key steps due to their extremely low concentration in peripheral blood. Herein, magnetic nanospheres (MNs) were fabricated by a convenient and highly controllable layer-by-layer assembly method. The MNs were nanosized with fast magnetic response, and nearly all of the MNs could be captured by 1 min attraction with a commercial magnetic scaffold. In addition, the MNs were very stable without aggregation or precipitation in whole blood and could be re-collected nearly at 100% in a monodisperse state. Modified with anti-epithelial-cell-adhesion-molecule (EpCAM) antibody, the obtained immunomagnetic nanospheres (IMNs) successfully captured extremely rare tumor cells in whole blood with an efficiency of more than 94% via only a 5 min incubation. Moreover, the isolated cells remained viable at 90.5 +/- 1.2%, and they could be directly used for culture, reverse transcription polymerase chain reaction (RT-PCR), and immunocytochemistry (ICC) identification. ICC identification and enumeration of the tumor cells in the same blood samples showed high sensitivity and good reproducibility. Furthermore, the IMNs were successfully applied to the isolation and detection of CTCs in cancer patient peripheral blood samples, and even one CTC in the whole blood sample was able to be detected, which suggested they would be a promising tool for CTC enrichment and detection.
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