Journal
ACS NANO
Volume 7, Issue 2, Pages 1379-1387Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn305030g
Keywords
DNA; nanotechnology; polymer; nuclease; resistance
Categories
Funding
- ARO [W911NF-11-1-0264]
- AFOSR [FA9550-11-1-0105]
- NIH [NIBIB-1R01EB011633, DP20D008724, DA031591]
- Henry and Camille Dreyfus Foundation
- UCSD Cryo-Electron Microscopy Facility (NIH, Agouron)
- UM Light Microscopy Facility (P30) [NSO47101]
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Herein, we describe a polymeric micellar nanoparfide capable of rendering nucleic acids resistant to nuclease digestion. This approach relies on utilizing DNA as the polar headgroup of a DNA polymer amphiphile in order to assemble well-defined, discrete nanoparticles. Dense packing of DNA in the micelle corona allows for hybridization of complementary oligonucleotides while prohibiting enzymatic degradation. We demonstrate the preparation, purification, and characterization of the nanoparticles, then describe their resistance to treatment with endo- and exonucleases induding snake-venom phosphodiesterase (SVP), a common, general DNA digestion enzyme.
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