Journal
ACS NANO
Volume 7, Issue 4, Pages 3362-3372Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn400171w
Keywords
nanoparticles; MRI; miRNA; cell tracking; ischemic diseases
Categories
Funding
- Marie Curie-Reintegration Grant [FP7-People-2007-4-3-IRG, 230929]
- MIT-Portugal program
- British Heart Foundation Programme Grant [RG/07/004/22659]
- FCT [PTDC/CTM/099659/2008, SFRH/BD/33466/2008]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/33466/2008, PTDC/CTM/099659/2008] Funding Source: FCT
- British Heart Foundation [PS/02/002/14893, RG/07/004/22659] Funding Source: researchfish
Ask authors/readers for more resources
Herein, we report the use of biodegradable nanoparticles (NPs) containing perfluoro-1,5-crown ether (PFCE), a fluorine-based compound (NP170-PFCE) with the capacity to track cells in vivo by magnetic ressonance imaging (MRI) and efficiently release miRNA. NP170-PFCE complexed with miRNAs accumulate whitin the cell's endolysosomal compartment and interact with higher frequency with argonaute2 (Ago2) and GW182 proteins, which are involved in the biological action of miRNAs, than commercial complexes formed by commercial reagents and miRNA, which in turn accumulate in the cell cytoplasm. The release of miRNA132 (miR132) from the NPs increased 3-fold the survival of endothelial cells (ECs) transplanted in vivo and 33-fold the blood perfusion in ischemic limbs relatively to control.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
