Journal
ACS NANO
Volume 7, Issue 6, Pages 4774-4784Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn400907f
Keywords
inclusion bodies; protein nanoparticles; cell guidance; 2D engineering; fibroblasts; microcontact printing; tissue engineering
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Funding
- DGI Grant POMAs [CTQ2010-19501]
- MICIN Project [BFU2010-17450]
- AGAUR [SGR2009-516, 2009SGR-108]
- Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN)
- Programa Personal de Tecnico de Apoyo (Modalidad Infraestructuras cientifico-tecnologicas, MICINN)
- Consejo Superior de Investigaciones Cientificas (CSIC)
- UAB
- ICREA ACADEMIA award
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Cell responses, such as positioning, morphological changes, proliferation, and apoptosis, are the result of complex chemical, topographical, and biological stimuli. Here we show the macroscopic responses of cells when nanoscale profiles made with inclusion bodies (IBs) are used for the 2D engineering of biological interfaces at the microscale. A deep statistical data treatment of fibroblasts cultivated on supports patterned with green fluorescent protein and human basic fibroblast growth factor-derived IBs demonstrates that these cells preferentially adhere to the IB areas and align and elongate according to specific patterns. These findings prove the potential of surface patterning with functional IBs as protein-based nanomaterials for tissue engineering.
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