Journal
ACS NANO
Volume 6, Issue 7, Pages 5757-5766Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn300842a
Keywords
injectable polyplex hydrogel; local delivery; sustained polyplex release; long-term therapy; siRNA
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Funding
- Korea Institute of Science and Technology
- Ministry of Education, Science and Technology of Korea
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Here, we describe a concept for localized and long-term delivery of short interfering RNA (siRNA) using an injectable polyplex hydrogel possessing thermosensitivity and biodegradability properties. We prepared a low molecular weight polyethyleneimine-poly(organophosphazene) conjugate as a thermosensitive and cationic polymer that has a cleavable ester linkage. The conjugates formed about 100 nm sized polyplexes with siRNAs, and the polyplex solution turned into a polyplex hydrogel at body temperature via a hydrophobic interaction. We injected the polyplex hydrogel with siRNA of cyclin B1, an essential protein for controlling the cell cycle, into the tumor xenograft model. Polyplexes were slowly released from the polyplex hydrogel by dissolution and degradation, allowing an in vivo antitumor effect via cyclin B1 gene silencing for 4 weeks with only a single injection.
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