Journal
ACS NANO
Volume 6, Issue 12, Pages 10992-10998Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn304599d
Keywords
dynamic nanostructures; DNA self-assembly; gold nanoparticles; cryo-electron microscopy; electrophoresis
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Funding
- Agence Nationale de le Recherche [ANR-11-JS10-0002]
- CNRS program Interface Physique Chimie Biologie: Soutien a la prise de risque
- Region Ile-de-France in the framework of C'Nano IdF
- CNRS
- CEA
- MESR
- Region Iie-de-France
- Fondation Pierre-Gilles de Gennes pour la Recherche
- Agence Nationale de la Recherche (ANR) [ANR-11-JS10-0002] Funding Source: Agence Nationale de la Recherche (ANR)
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We produce gold nanoparticle dimers with a surface-to-surface distance that varies reversibly by a factor of 3 when hybridizing or removing a single target DNA strand. The dimers are built on one DNA template that features a stem-loop enabling the interparticle distance change. Using electrophoresis, we reach 90% sample purities and demonstrate that this chemical process is reversible in solution at room temperature for a low molar excess of the target DNA strand. The kinetics of the reaction is asymmetric due to steric hindrance in the stem-loop opening process. Furthermore, a statistical analysis of cryo-electron microscopy measurements allows us to provide the first quantitative analysis of distance changes in chemically switchable nanoparticle assemblies.
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