Journal
ACS NANO
Volume 6, Issue 10, Pages 8692-8701Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn302270b
Keywords
tobacco mosaic virus; artificial enzyme; self-asselmbly; nanodisk; nanotube; glutathione peroxidase
Categories
Funding
- Natural Science Foundation of China [21234004, 91027023, 20921003, 21004028]
- 111 project [B06009]
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Construction of catalytic centers on natural protein aggregates is a challenging topic in biomaterial and biomedicine research. Here we report a novel construction of artificial nanoenzyme with glutathione peroxidase (GPx)-like function. By engineering the surface of tobacco mosaic virus (TMV) coat protein, the main catalytic components of GPx were fabricated on TMV protein monomers. Through direct self-assembly of the functionalized viral coat proteins, the multi-GPx centers were installed on these well-defined nanodisks or nanotubes. With the help of muti-selenoenzyme centers, the resulting organized nanoenzyme exhibited remarkable GPx activity, even approaching the level of natural GPx. The antioxidation study on subcell mitochondrial level demonstrated that virus-based nanoenzyme exerted excellent capacity for protecting cell from oxidative damage. This strategy represents a new way to develop artificial nanoenzymes.
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