Journal
ACS NANO
Volume 6, Issue 10, Pages 8803-8814Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn302633q
Keywords
mismatch self-dimer; hairpin; DNA/AgNC probe; miRNA detection; fluorescence; silver nanoclusters; native gel electrophoresis
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Funding
- Center for Synthetic Biology at Copenhagen University
- UNIK research initiative of the Danish Ministry of Science, Technology and Innovation [09-065274]
- Danish Council for Independent Research-Natural Sciences
- Fonds voor Wetenschappelijk Onderzoek FWO [G.0402.09, G.0413.10, G.0697.11]
- K. U. Leuven Research Fund [GOA 2011/03]
- Flemish government [CASAS METH/08/04]
- Federal Science Policy of Belgium [IAP-VI/27]
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The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated. The findings show that especially the propensity for mismatch self-dimer formation of the DNA probes can be a good indicator for the creation and stabilization of red emissive AgNCs. Also, the role of the thermal stability of the secondary DNA structures (mismatch self-dimer and hairpin monomers) and the observed AgNC red emission intensity were investigated. These findings can form the basis for a rationale to design new red emissive AgNC-based probes. As an example, a bright red emissive AgNC-based DNA probe was designed for RNA-miR172 detection. The latter opens the possibility to create a variety of AgNC-based DNA probes for the specific detection of plant and animal miRNAs.
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