Highly Sensitive Electrochemiluminescent Nanobiosensor for the Detection of Palytoxin

Marine toxins appear to be increasing in many areas of the world. An emerging problem in the Mediterranean Sea is represented by palytoxin (PITX), one of the most potent marine toxins, frequently detected in seafood. Due to the high potential for human toxicity of PITX, there is a strong and urgent need for sensitive methods toward its detection and quantification. We have developed an ultrasensitive electrochemiluminescence-based sensor for the detection of PITX, taking advantage of the specificity provided by anti-PITX antibodies, the good conductive properties of carbon nanotubes, and the excellent sensitivity achieved by a luminescence-based transducer. The sensor was able to produce a concentration-dependent light signal, allowing PITX quantification in mussels, with a limit of quantification (LOQ = 2.2 mu g/kg of mussel meat) more than 2 orders of magnitude more sensitive than that of the commonly used detection techniques, such as LC-MS/MS.