Journal
ACS NANO
Volume 6, Issue 9, Pages 7928-7934Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn3023624
Keywords
mammalian chromatin; single molecule; soft lithography; molecular stretching; fluorescence imaging; AFM
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Funding
- Cornell Center on the Microenvironment & Metastasis from the National Cancer Institute [CA143876]
- National Institutes of Health [DA025722]
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Individual chromatin molecules contain valuable genetic and epigenetic information. To date, there have not been reliable techniques available for the controlled stretching and manipulation of individual chromatin fragments for high-resolution imaging and analysis of these molecules. We report the controlled stretching of single chromatin fragments extracted from two different cancerous cell types (M091 and HeLa) characterized through fluorescence microscopy and atomic force microscopy (AFM). Our method combines soft lithography with molecular stretching to form ordered arrays of more than 250 000 individual chromatin fragments immobilized into a beads-on-a-string structure on a solid transparent support. Using fluorescence microscopy and AFM, we verified the presence of histone proteins after the stretching and transfer process.
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