Journal
ACS NANO
Volume 5, Issue 7, Pages 5478-5489Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn2007496
Keywords
gold nanoparticles; transferrin; uptake; endocytosis; aggregation; cytotoxicity; TEM
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada (BiopSys Network) [NETGP35015-07, RGPIN 288231-09]
- Canadian Insitute of Health Research [MOP-93532]
- Canadian Foundation for Innovation
- Ministry of Research and Innovation in Ontario
- Ontario Graduate Scholarship
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Aggregation appears to be a ubiquitous phenomenon among all nanoparticles and Its influence In mediating cellular uptake and Interactions remain unclear. Here we developed a simple technique to produce transferrin-coated gold nanoparticle aggregates of different sizes and characterized their uptake and toxicity In three different cell lines. While the aggregation did not elicit a unique toxic response, the uptake patterns were different between single and aggregated nanoparticles. There was a 25% decrease in uptake of aggregated nanoparticles with HeLa and A549 cells In comparison to single and monodisperse nanoparticles. However, there was a 2-fold increase in MDA-MB 435 cell uptake for the largest synthesized aggregates. These contrasting results suggest that cell type and the mechanism of interactions may play a significant role. This study highlights the need to investigate the behavior of aggregates with cells on a case-by-case basis and the importance of aggregation in mediating targeting and intracellular trafficking.
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