Journal
ACS NANO
Volume 6, Issue 1, Pages 441-450Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn203769e
Keywords
nanopore; graphene; DNA; translocation; sequencing
Categories
Funding
- National Institutes of Health [R21 CA155863]
- Office of Naval Research (ONR)
- Midwestern Cancer Nanotechnology Training Center at UIUC [NIH-NCI R25 CA154015]
- NSF [0915718]
- NDSEG
- NIH [R21 CA155863]
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [0915718] Funding Source: National Science Foundation
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We report the development of a multilayered graphene-Al2O3 nanopore platform for the sensitive detection of DNA and DNA-protein complexes. Graphene-Al2O3 nanolaminate membranes are formed by sequentially depositing layers of graphene and Al2O3, with nanopores being formed in these membranes using an electron-beam sculpting process. The resulting nanopores are highly robust, exhibit low electrical noise (significantly lower than nanopores in pure graphene), are highly sensitive to electrolyte pH at low KCl concentrations (attributed to the high buffer capacity of Al2O3), and permit the electrical biasing of the embedded graphene electrode, thereby allowing for three terminal nanopore measurements. In proof-of-principle biomolecule sensing experiments, the folded and unfolded transport of single DNA molecules and RecA-coated DNA complexes could be discerned with high temporal resolution. The process described here also enables nanopore integration with new graphene-based structures, Including nanoribbons and nanogaps, for single-molecule DNA sequencing and medical diagnostic applications.
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