Journal
ACS NANO
Volume 5, Issue 6, Pages 4858-4864Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn2009485
Keywords
gold nanorod; surface plasmon resonance; biosensor; TNF-alpha
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Funding
- John R. Oishei Foundation
- International Joint Research Center for Nanophotonics and Biophotonics
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In this study, we report the development of a nanoparticle-enhanced biosensor by Integrating both the nanoparticles and immunoassay sensing technologies into a phase interrogation surface plasmon resonance (SPR) system for detecting antigen at a concentration as low as the femtomolar range. Our work has demonstrated that the plasmonic field extension generated from the gold film to gold nanorod (GNR) has led to a drastic sensitivity enhancement. Antibody-functionalized sensing film, together with antibody-conjugated GNRs, was readily served as a plasmonic coupling partner that can be used as a powerful ultrasensitive sandwich immunoassay for cancer-related disease detection. Experimentally, it was found that the bioconjugated GNR labels enhance the tumor necrosis factor alpha (TNF-alpha.) antigen signal with more than 40-fold increase compared to the traditional SPR biosensing technique. The underlying principle was analyzed by simulating the near-field coupling between the sensing film and the GNR. The results have shown that GNRs were readily served as promising amplification labels in SPR sensing technology.
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