Journal
ACS NANO
Volume 6, Issue 1, Pages 141-150Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn2031353
Keywords
influenza virus; quantum dot; infection; single-particle tracking; endocytosis; bionanotechnology
Categories
Funding
- National Basic Research Program of China (973 Program) [2011CB933600, 2006CB933100]
- NSFC [20921062, 20621502, 20833006, 21005056]
- Administrative Committee of East Lake Hi-Tech Development Zone [[2011]137]
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Exploring the virus Infection mechanisms is significant for defending against virus infection and providing a basis for studying endocytosis mechanisms. Single-particle tracking technique is a powerful tool to monitor virus infection in real time for obtaining dynamic information. In this study, we reported a quantum-dot-based single-particle tracking technique to efficiently and globally research the virus infection behaviors In Individual cells. It was observed that many influenza viruses were moving rapidly, converging to the microtubule organizing center (MTOC), interacting with acidic endosomes, and finally entering the target endosomes for genome release, which provides a vivid portrayal of the five-stage virus infection process. This report settles a long-pending question of how viruses move and interact with acidic endosomes before genome release in the perinuclear region and also finds that influenza virus infection Is likely to be a MTOC rescue model for genome release. The systemic technique developed in this report Is expected to be widely used for studying the mechanisms of virus Infection and uncovering the secrets of endocytosis.
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