Journal
ACS NANO
Volume 5, Issue 4, Pages 3166-3171Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn2003053
Keywords
carbonaceous materials; silver nanoparticles; chromogenic detection; thiol-containing biomolecules; protein
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Funding
- National Basic Research Program of China [2010CB934700]
- National Natural Science Foundation of China [91022032, 50732006]
- International Science & Technology Cooperation Program of China [2010DFA41170]
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Thiol-containing biomolecules show strong affinity with noble metal nanostructures and could not only stably protect them but also control the self-assembly process of these special nanostructures. A highly selective and sensitive chromogenic detection method has been designed for the low and high molecular weight thiol-containing biomolecules, including cysteine, glutathione, dithiothreitol, and bovine. serum albumin, using a new type of carbonaceous nanospheres loaded with silver nanoparticles. (Ag NPs) as carrier. This strategy relies upon the place-exchange process between the reporter dyes on the surface of Ag NPs and the thiol groups of thiol-containing biomolecules. The concentration of biomolecules can be determined by monitoring with the fluorescence Intensity of reporter dyes dispersed In solution. This new chromogenic assay method could selectively detect these biomolecules in the presence of various other amino acids and monosaccharides and even sensitively detect the thiol-containing biomolecules with different molecular weight, even including proteins.
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