Journal
ACS NANO
Volume 5, Issue 12, Pages 9977-9983Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn203745n
Keywords
solid lipid nanoparticles; siRNA; sustained release; drug delivery
Categories
Funding
- National Science Foundation [CBET-0827806]
- NIH/NIAMS [RC2ARO58955]
- Knut and Alice Wallenberg Foundation, Sweden
- PC Project
Ask authors/readers for more resources
Small interfering RNA (siRNA) Is a highly potent drug in gene-based therapy with a challenge of being delivered In a sustained manner. Nanoparticle drug delivery systems allow for Incorporating and controlled release of therapeutic payloads. We demonstrate that solid lipid nanoparticles can incorporate and provide sustained release of siRNA. Tristearin solid lipid nanoparticles, made by nanoprecipitation, were loaded with siRNA (4.4-5.5 wt % loading ratio) using a hydrophobic ion pairing approach that employs the cationic lipid DOTAP. Intradermal Injection of these nanocarriers in mouse footpads resulted In prolonged siRNA release over a period of 10-13 days. In vitro cell studies showed that the released siRNA retained its activity. Nanoparticles developed in this study offer an alternative approach to polymeric nanoparticles for encapsulation and sustained delivery of siRNA with the advantage of being prepared from physiologically well-tolerated materials.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available