Journal
ACS NANO
Volume 4, Issue 6, Pages 3254-3258Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn100244a
Keywords
nanothermometer; upconversion; HeLa cancer cell; nanoparticles; thermal sensing
Categories
Funding
- Universidad Autonoma de Madrid
- Spanish Ministerio de Educacion y Ciencia [MAT 2007-64686]
- Banco Santander-CEAL-UAM
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- Gouvernement du Quebec, Ministere du Developpement economique, de l'Innovation et de l'Exportation
- Spanish Minsterio de Educacion [PR2009-0040]
- Comunidad Autonoma de Madrid [CCG087-UAM/MAT-4434, S2009/MAT-1756]
Ask authors/readers for more resources
Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF4:Er3+,Yb3+ nanoparticles, where the intensity ratio of the green fluorescence bands of the Er3+ dopant ions (H-2(11/2) -> I-4(15/2) and S-4(3/2) -> I-4(15/2)) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF4:Er3+,Yb3+ nanoparticles in water using a pump-probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 degrees C to its thermally induced death at 45 degrees C.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available