Journal
ACS NANO
Volume 4, Issue 9, Pages 5047-5054Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn100773q
Keywords
planar lipid bilayers; polyelectrolyte multilayers; melittin; single channel monitoring; impedance spectroscopy
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Funding
- EU
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A lipid bilayer with gigaohm resistance was fabricated over a single 800 nm pore in a Si3N4 chip using 50 nm liposomes. The nanopore was prefilled with a polyelectrolyte multilayer (PEM) that triggered the spontaneous fusion of the lipid vesicles. Pore-forming peptide melittin was incorporated in the bilayer, and single channel activities were monitored for a period of 2.5 weeks. The long lifetime of the system enabled the observation of the time-dependent stabilization effect of the melittin open state upon bias application.
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