Journal
ACS NANO
Volume 5, Issue 1, Pages 360-366Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn101740e
Keywords
mesoporous polymer-silica composite; endocytosis; cellular uptake; drug delivery; mesostructured nanoparticles; controlled release
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Funding
- U.S. DOE Ames Laboratory through the office of Basic Energy Sciences [DE-AC02-07CH11358]
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A two-dimensional hexagonal ordered mesoporous polymer-silica hybrid nanoparticle (PSN) material was synthesized by polymerization of acrylate monomers on the surface of SBA-15 mesoporous silica nanoparticles. The structure of the PSN material was analyzed Using a series of different techniques, Including transmission electron microscopy, powder X-ray. diffraction, and N-2 sorption analysis. These structurally ordered, mesoporous polymer-silica hybrid nanoparticles were used for the controlled release Of membrane impermeable macromolecules inside eukaryotic cells. The cellular uptake efficiency and biocompatibility of PSN with human cervical cancer cells (HeLa) were investigated : Our results show that the inhibitory, concentration (IC50) of PSN is very high (>100 mu g/mL per million cells), while the median effective concentration for the uptake (EC50) of PSN is low (EC50 = 44 mu g/mL), indicating that PSNs are fairly biocompatible and easily up taken in vitro. A membrane impermeable macromolecule, 40 kDa FITC-Dextran, was loaded into the mesopores of PSNs at low pH: We demonstrated that the PSN material could indeed serve as a transmembrane carrier for the controlled release of FITC-Dextran at the pH level inside live HeLa cells. We believe that further developments of this PSN material will lead to a new generation of nanodevices for intracellular controlled delivery applications.
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