Journal
ACS NANO
Volume 4, Issue 12, Pages 7467-7474Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn102644u
Keywords
pH-responsive nanoprobe; Forster resonance energy transfer; ratiometric imaging; dual emission; associating polyelectrolyte; nanostructure transformation
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Funding
- National Science Council (NSC), Taiwan [98-2120-M-007-007]
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A dual-emission nanoprobe that can sense changes in the environmental pli is designed based on the concept of pH-responsive Forster resonance energy transfer induced by the conformational transition of an associating polyelectrolyte, N-palmitoyl chitosan, bearing a donor (Cy3) or an acceptor (Cy5) moiety. We demonstrate that the developed pH-responsive nanoprobe can be used to ratiometrically image and thus discriminate the pH changes In the biological environment at different length scales.
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