Journal
ACS NANO
Volume 4, Issue 9, Pages 5505-5511Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn101638u
Keywords
gold nanoparticles; charge-reversal polyelectrolyte; drug delivery; layer-by-layer assembly; siRNA delivery
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Funding
- National Natural Science Foundation of China [30970784, 30772007]
- National Key Basic Research Program of China [2009CB930200]
- Chinese Academy of Sciences (CAS) [07165111ZX]
- China-Finland Nanotechnology [2008DFA01510]
- Tianjin Natural Science Foundation [09JCYB-JC13800]
- Tianjin University Youth Teacher Culture Foundation [TJU-YFF-08B15]
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Charge-reversal functional gold nanoparticles first prepared by layer-by-layer technique were employed to deliver small interfering RNA (siRNA) and plasmid DNA into cancer cells. Polyacrylamide gel electrophoresis measurements of siRNA confirmed the occurrence of the charge-reversal property of functional gold nanoparticles. The expression efficiency of enhanced green fluorescent protein (EGFP) was improved by adjuvant transfection with charge-reversal functional gold nanoparticles, which also had much lower toxicity to cell proliferation. Lamin A/C, an important nuclear envelope protein, was effectively silenced by lamin A/C-siRNA delivered by charge-reversal functional gold nanoparticles, whose knockdown efficiency was better than that of commercial Lipofectamine 2000. Confocal laser scanning microscopic images indicated that there was more cy5-siRNA distributed throughout the cytoplasm for cyanine 5-siRNA/polyethyleneimine/cis-aconitic anhydride-functionalized poly(allylamine)/polyethyleneimine/11-mercaptoundecanoic acid-gold nanoparticle (cy5-siRNA/PEI/PAH-Cit/PEI/MUA-AuNP) complexes. These results demonstrate the feasibility of using charge-reversal functional gold nanoparticles as a means of improving the nucleic acid delivery efficiency.
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