Journal
ACS NANO
Volume 5, Issue 1, Pages 493-499Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn102344t
Keywords
carbon nanotubes; functionalization; plant cell; catharanthus protoplasts; endosome escaping; organelle targeting
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Funding
- New Energy and Industrial Technology Development Organization (NEDO) of Japan
- Japan Society for the Promotion of Science (JSPS)
- Centre National de la Recherche Scientifique
- European Union [NMP4-CT-2006-031847]
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Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a., high cytoplasmic concentration by circumventing entrapment into endosomes and other lytic organelles. Motivated by such aim, we have investigated the capability of multiwalled carbon nanotubes (MWCNTs) to penetrate the cell membrane of plant protoplasts (plant cells made devoid of their cell walls via enzymatic, treatment) and studied their internalization mechanism via confocal imaging and TEM techniques. Our results indentified an endosome-escaping uptake mode of MWCNTs by plant protoplasts. Moreover, short MWCNTs (<100. nm) were observed to target specific cellular substructures including the nucleus, plastids, and vacuoles. These, findings are expected to have a significant impact on plant cell biology and transformation technologies.
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